Method of the real-time RT-PCR for the detection of nipah virus（NiV）(50 reactions)

【Precautions】
1. The laboratory should be divided into at least three areas: sample processing area, reaction mixture preparation area, and detection area.
2. Items in each area should be used exclusively and not cross-used to avoid contamination. After detection, the workbench should be cleaned immediately.
3. When aliquoting the reaction solution, try to avoid generating bubbles. Before running the machine, check whether each reaction tube is properly capped to prevent leakage of fluorescent substances and contamination of the instrument.
4. The positive control should be vigorously shaken on a micro vortex mixer for 5–10 seconds before being aspirated.
5. Components in the kit should avoid repeated freeze-thaw cycles (less than five times).
【Specification】50 tests/box
【Storage】Fluorescent RT-PCR Detection Kit – store at -20°C.
Accuracy and stability, comparative evaluation
Method of the real-time RT-PCR for the detection of nipah virus（NiV）(50 reactions)
【Precautions】
1. The laboratory should be divided into at least three areas: sample processing area, reaction mixture preparation area, and detection area.
2. Items in each area are for dedicated use only and must not be cross-used to avoid contamination. After detection, the workbench should be cleaned immediately.
3. When aliquoting reaction solution, avoid generating bubbles as much as possible. Before use on the instrument, check whether each reaction tube is tightly capped to prevent fluorescent substances from leaking and contaminating the instrument.
4. Positive controls should be vigorously vortexed for 5–10 seconds before aspirating.
5. Components of the kit should avoid repeated freeze-thaw cycles (less than five times).
【Specification】 50 tests/box
【Storage】 Store the fluorescent RT-PCR detection kit at -20℃.

Accuracy and stability, comparative evaluation, evaluation started from 2023.